Fig. 6: Macrophage isolation confirmed the direct effect of EZH2 in CaOx crystal-induced MMT in kidney. | Communications Biology

Fig. 6: Macrophage isolation confirmed the direct effect of EZH2 in CaOx crystal-induced MMT in kidney.

From: EZH2-mediated macrophage-to-myofibroblast transition contributes to calcium oxalate crystal-induced kidney fibrosis

Fig. 6

A Schematic diagram of macrophage isolation, Created in BioRender. Yuqi, X. (2025) https://BioRender.com/a33h147. B Gating strategy of flow cytometry. The expression of α-SMA in isolated macrophages was detected. C Expression of EZH2 was detected by immunoblotting (n = 3). D Expression of Collagen I and fibronectin was detected by immunoblotting (n = 3). Data are presented as the mean ± SEM. *p < 0.05 versus EZH2fl/fl group; #p < 0.05 versus CaOx EZH2fl/fl group. P values were determined by the two-tailed Student’s t test.

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