Fig. 7: Knockdown of Ezh2 decreases CaOx crystal-induced MMT cells in Vitro. | Communications Biology

Fig. 7: Knockdown of Ezh2 decreases CaOx crystal-induced MMT cells in Vitro.

From: EZH2-mediated macrophage-to-myofibroblast transition contributes to calcium oxalate crystal-induced kidney fibrosis

Fig. 7

A Representative pictures of COM-treated macrophages. B Two-color immunofluorescence identifies MMT cells that coexpress macrophage (F4/80, green) and myofibroblast (a-SMA, red) markers in the RAW264.7 cell line (n = 5). C The proportion of MMT cells in the COM-treated macrophages was detected by flow cytometry (n = 3). D EZH2, Collagen I, α-SMA and fibronectin were expressed by immunoblotting in the RAW264.7 cell line (n = 3). Data are presented as the mean ± SEM. *p < 0.05 versus shNC group; #p < 0.05 versus COM-treated shNC group. P values were determined by the two-tailed Student’s t test.

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