Fig. 9: Pharmacological inhibition of EZH2 by GSK-126 ameliorates MMT-meditated kidney fibrosis in nephrocalcinosis. | Communications Biology

Fig. 9: Pharmacological inhibition of EZH2 by GSK-126 ameliorates MMT-meditated kidney fibrosis in nephrocalcinosis.

From: EZH2-mediated macrophage-to-myofibroblast transition contributes to calcium oxalate crystal-induced kidney fibrosis

Fig. 9

A Renal function and body weight were measured on day 12 of modeling (n = 5). Representative pictures of HE staining, Sirus rad staining (B), and Von Kossal staining (C) (n = 5). D Two-color immunofluorescence identifies MMT cells in nephrocalcinosis mice (n = 5). E EZH2, Collagen I, α-SMA and fibronectin were expressed by immunoblotting in COM-treated macrophages (n = 3). F Two-color immunofluorescence identifies MMT cells in the RAW264.7 cell line (n = 5). G EZH2, Collagen I, α-SMA and fibronectin were expressed by immunoblotting in nephrocalcinosis mice (n = 3). Data are presented as the mean ± SEM. *p < 0.05 versus control group; #p < 0.05 versus CaOx nephrocalcinosis group. P values were determined by the two-tailed Student’s t test.

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