Fig. 1: Effector-triggered immunity (ETI) to Pseudomonas syringae pv. tomato (Pst) in Arabidopsis is associated with DCL1 and increased nuclear-localized tRNAs.
From: A nuclear tRNA-derived fragment triggers immunity in Arabidopsis
a Bacterial growth in wild type (WT) and dcl1-7 at 0 and 3 day-post-infection (dpi) with Pst containing or lacking AvrRpt2 (Pst_avrRpt2 or Pst, respectively); rps2 is an ETI control. The mean±RSE (n ≥ 9) from three independent experiments are presented. b Relative PR1 transcription levels measured by qRT-PCR are presented as mean±SD (n = 3). c Size distribution of nuclear sRNAs (11–100 nts) in WT and dcl1-7 at 1 h-post-infection (hpi) with Pst_avrRpt2 or buffer (mock); the y-axis represents a normalized read count in RPM. d Percentage of tRNA-aligned reads in 71–100 nts sRNAs. e Normalized read counts of tRNA-derived 71–100 nts RNAs as mean±SE (n = 3). Significance between indicated pairs is noted; *P < 0.05; **P < 0.01; ***P < 0.001 (t-test).
