Fig. 2: AGO2-associated 5′-tRFs display DCL1-dependent induction in Arabidopsis.
From: A nuclear tRNA-derived fragment triggers immunity in Arabidopsis
a–c sRNA-seq analysis of nuclear 5′-tRFs in Pst_avrRpt2- or mock-inoculated WT and dcl1-7 at 1 hpi. d–f Nuclear AGO-associated 5′-tRFs in Pst_avrRpt2-, Pst-, or mock-inoculated HA-AGO1 and HA-AGO2 transgenic Arabidopsis at 1 hpi were identified by sRIP-seq via α-HA; WT was the negative control. The y-axis represents a normalized read count in RPM. Size distribution of 5′-tRFs (a, d). Normalized read counts of 31 nt-long 5′-tRFs originated from each tRNA isodecoder (b, e) and of a representative Pst_avrRpt2-inducible, DCL1-dependent 5′-tRF (tRF31Asp2) (c, f). The mean ± SE is presented (n = 3, a–c; n = 2, d–f). Significance from mock noted for highlighted samples only; *P < 0.05; **P < 0.01; ***P < 0.001 (t-test).
