Fig. 1: Phylogenetic analysis of actinobacterial chaperonins. | Communications Biology

Fig. 1: Phylogenetic analysis of actinobacterial chaperonins.

From: Genetic and structural insights into the functional importance of the conserved gly-met-rich C-terminal tails in bacterial chaperonins

Fig. 1

a Phylogenetic relationships between 325 actinobacterial chaperonin sequences were inferred using a neighbourhood-joining algorithm and presented as a phylogenetic tree. The phylogenetic tree shows three clades. The yellow-coloured branch represents the E. coli GroEL sequence, which was included in the alignment. b Site specific conservation rates were calculated using ConSurf’s Rate4Site algorithm from the multiple sequence analysis and mapped onto E. coli GroEL sequence. The graph represents the normalized conservation scores (lowest score represents conservation, with a standard deviation of one) as a function of GroEL’s primary sequence. The domain regions of the equatorial (E), intermediate (I) and apical (A) domains and the CTS region (C) are indicated. c Sequence Logo depicting the diversity in the CTS region of the actinobacterial chaperonins, starting with the conserved Proline. d Scatter plot showing hydrophobicity of the chaperonin CTSs (GRAVY Scores) as a function of their average charges (pI). Each dot represents one CTS and the spots are colour coded in the same way as the three branches in the phylogenetic tree. A detailed phylogenetic tree with taxon names and node ages is presented in the supplementary information.

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