Table 1 E. coli Strains and Plasmid Vectors used in this Study
Name | Description | Source or Reference |
|---|---|---|
Strains | ||
E. coli AI90 | groEL deletion strain - groEL on a p15A camR shelter plasmid is regulated by Plac promoter | |
E. coli LG6 | Chromosomal groES/L operon under lactose/IPTG inducible Plac promoter | |
E. coli MGM100 | Chromosomal groES/L operon under arabinose inducible PBAD promoter | |
E. coli SV2 | B178 derived temperature sensitive strain having groEL44 allele encoding GroEL E191G | |
E. coli A7579 | C600 derived temperature sensitive strain having groEL100 allele encoding GroEL S201F | |
Plasmid Vectors | ||
pBAD24 | L-arabinose inducible expression vector, ColE1 origin, AmpR. | |
pTrc99A | Ptac based expression vector, ColE1 origin AmpR. | |
pBAD/GSL | E. coli groESL operon cloned in pBAD24 | |
pTrc/GSL | E. coli groESL operon cloned in pTrc99A | |
pBAD/GroELΔC13 | pBAD24 harbouring the ORF for GroELΔC13. | This study |
pBAD/AAM | pBAD24 harbouring the ORF for E. coli GroEL with (AAM)4M C-terminus | This study |
pBAD/GGD | pBAD24 harbouring the ORF for E. coli GroEL with (GGD)4D C-terminus | This study |
pBAD/PPM | pBAD24 harbouring the ORF for E. coli GroEL with (PPM)4M C-terminus | This study |
pTrc/GroELΔC13 | pTrc99a harbouring ORF for GroELΔC13 | This study |
pTrc/AAM | pTrc99a harbouring the ORF for E. coli GroEL with (AAM)4M C-terminus | This study |
pTrc/GGD | pTrc99a harbouring the ORF for E. coli GroEL with (GGD)4D C-terminus | This study |
pTrc/PPM | pTrc99a harbouring the ORF for E. coli GroEL with (PPM)4M C-terminus | This study |
