Fig. 1: GC induced apoptosis of OBs. | Communications Biology

Fig. 1: GC induced apoptosis of OBs.

From: Glucocorticoids regulate the expression of Srsf1 through Hdac4/Foxc1 axis to induce apoptosis of osteoblasts

Fig. 1

A, B ALP detection for OBs (n = 4). C, D TUNEL/DAPI staining detection of OBs TUNEL* cell number (n = 3). E, F Apoptosis detected by Annexin V-FITC/PI staining (n = 5). G–I The expression levels of cleaved-CASP3 and cleaved-CASP9 were detected by Western Blot (n = 3). J–P Western Blot was used to detect the expression of BCL2, BAD, BMF, BAX, CYT C, and BCL-XL (n = 3). Data were expressed as mean ± standard deviation in (B, D, F, H, I, K–P), where statistical significance was calculated using Student’s t test in (K–P) and using one-way analysis of variance (ANOVA) with Tukey post hoc test in (B, D, F, H, I). *P < 0.05, **P < 0.01, ***P < 0.001, nsP > 0.05. 0.0: 0.0 mg/mL, 0.1: 0.1 mg/mL, 0.2: 0.2 mg/mL, 0.3: 0.3 mg/mL, 0.4: 0.4 mg/mL.

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