Fig. 2: Screening and identification of splicing factors associated with GC-induced OB apoptosis. | Communications Biology

Fig. 2: Screening and identification of splicing factors associated with GC-induced OB apoptosis.

From: Glucocorticoids regulate the expression of Srsf1 through Hdac4/Foxc1 axis to induce apoptosis of osteoblasts

Fig. 2

A Heat map showing differentially expressed genes (n = 3). B Gene ontology (GO) analysis (n = 3). C Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment pathway analysis (n = 3). D Heat map showing differentially expressed apoptosis-related genes (n = 3). E Heat map showing differentially expressed RNA splice-related genes (n = 3). F–K The relative expression of Srsf1, Srsf2, Srsf3, Srsf7, Bmf, and Bcl2l1 was detected by qPCR (n = 4). L–M The expression level of SRSF1 was detected by western blot (n = 4). N The correlation between SRSF1 expression and OB apoptosis rate was analyzed by Pearson correlation (n = 4). In (F–K, M), the data are expressed as mean ± SD, where statistical significance was calculated using Student’s t test in (F–K) and using one-way ANOVA with Tukey post hoc test in (M). Control control group, GC glucocorticoid treatment group. *P < 0.05, **P < 0.01, ***P < 0.001, nsP > 0.05.

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