Fig. 3: Effect of Srsf1 overexpression on OB apoptosis induced by GC. | Communications Biology

Fig. 3: Effect of Srsf1 overexpression on OB apoptosis induced by GC.

From: Glucocorticoids regulate the expression of Srsf1 through Hdac4/Foxc1 axis to induce apoptosis of osteoblasts

Fig. 3

A qPCR to verify Srsf1 overexpression efficiency (n = 4), GV492: lentiviral vector, OE-Srsf1: overexpression of Srsf1. B, C The overexpression efficiency of SRSF1 was detected by western blot (n = 3). D, E TUNEL/DAPI staining detection of OBs TUNEL* cell number (n = 3). F–H Expression levels of cleaved-CASP3 and cleaved-CASP9 were detected by western blot (n = 3). I, J Apoptosis detected by Annexin V-FITC/PI staining (n = 4). In (A, C, D, G, H, and J), the data were expressed as mean ± SD, and statistical significance was calculated using one-way ANOVA with Tukey post hoc test. *P < 0.05, **P < 0.01, ***P < 0.001, nsP > 0.05.

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