Fig. 5: SRSF1 regulates alternative splicing of Bmf pre-mRNA and participates in GC-induced OB apoptosis. | Communications Biology

Fig. 5: SRSF1 regulates alternative splicing of Bmf pre-mRNA and participates in GC-induced OB apoptosis.

From: Glucocorticoids regulate the expression of Srsf1 through Hdac4/Foxc1 axis to induce apoptosis of osteoblasts

Fig. 5

A qPCR was utilized to detect the expression changes in Bmf variants (n = 4). B, C Structure diagram of human and mouse BMF protein subtypes. D The knockdown efficiency of Bmf was detected by qPCR (n = 4), sh-Bmf: knockdown of Bmf. E, F Western Blot detection of BMF knockdown efficiency (n = 3). G, H Apoptosis detected by Annexin V-FITC/PI staining (n = 4). I, J TUNEL/DAPI staining detection of OBs TUNEL* cell number (n = 3). K–M Expression levels of cleaved-CASP3 and cleaved-CASP9 were detected by western blot (n = 3). In (A, D, F, H, I, L, M), the data were expressed as mean ± SD, and statistical significance was calculated using one-way ANOVA with Tukey post hoc test. *P < 0.05, **P < 0.01, ***P < 0.001, nsP > 0.05.

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