Fig. 6: FOXC1 binds to the Srsf1 promoter to regulate Srsf1 expression.

A Relative expression of Foxc1 detected by qPCR (n = 4). B, C Expression of FOXC1 was detected by western blot (n = 3). D CHIP-qPCR was utilized to detect the binding sites of FOXC1 and Srsf1 promoter region. E Relative expression of Foxc1 by qPCR (n = 4). F, G Western Blot was utilized to detect the expression of FOXC1 (n = 3). H Relative expression of Foxc1 by qPCR (n = 5). I, J The expression level of FOXC1 was detected by western blot (n = 3). K Relative expression of Srsf1 by qPCR (n = 4). L, M Western blot was utilized to detect the expression of SRSF1 (n = 3). N Relative expression of Srsf1 detected by qPCR (n = 4). O, P Western blot was utilized to detect the expression level of SRSF1 (n = 3). In (A, C, D, E, G, H, J, K, M, N, P), the data are expressed as mean ± SD, where statistical significance was calculated using Student’s t test in (A, C, D) and using one-way ANOVA with Tukey post hoc test in (F, G, I, J, L, M, O). ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, ^nsP > 0.05.