Fig. 7: HDAC4 suppressed Foxc1 expression by decreasing H3K9ac and H3K27ac levels in the Foxc1 promoter region.

A Relative expression of Foxc1 detected by qPCR (n = 5). B, C Expression of FOXC1 was detected by western blot (n = 3). D Relative expression of Hdac family members by qPCR (n = 5). E Relative expression of Hdac4 detected by qPCR (n = 5). F, G Western blot was utilized to detect the expression level of HDAC4 (n = 3). H CHIP-qPCR was utilized to detect H3K9ac and H3K27ac in the Foxc1 promoter region (n = 4). I Relative expression of Foxc1 detected by qPCR (n = 5). J, K The expression of FOXC1 was detected by western blot (n = 4). L Relative expression of Hdac4 detected by qPCR (n = 5). M, N Western blot was utilized to detect the expression of HDAC4 (n = 3). O CHIP-qPCR was utilized to detect H3K9ac and H3K27ac in the Foxc1 promoter region (n = 4). P Relative expression of Foxc1 detected by qPCR (n = 5). Q, R Western blot was utilized to detect the expression level of FOXC1 (n = 4). In (A, C, D, E, G, H, I, K, L, N, O, P, R), the data are expressed as mean ± SD, where statistical significance was calculated using Student’s t test in (A, C, D, O) and using one-way ANOVA with Tukey post hoc test in (E, G, H, I, K, L, N, P, R). ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, ^nsP > 0.05.