Table 1 Primer sequences
Gene name | Forward primer | Reverse primer |
|---|---|---|
Srsf1 | GTGGAAGCTGGCAGGACTTA | AGGCAGTTTCTCCCTCGTGA |
Srsf2 | GCCCGAAGATCCAAGTCCAA | GCTTGCCGATTCATCATTTTCT |
Srsf3 | AACCTTAGGTCCTGCCCAGT | CAGCAGCTCTTAAACACCGC |
Srsf6 | CTCGGTCCCCCAAAGAGAAC | ACATGGACCGAGCCTTTGAG |
Srsf9 | GAACGGGCCTCCTACAAGAC | CCATCCCCATTCCGTCCTTC |
Srsf10 | AGTAGACCGACTGGAAGACCA | TTGGTGTGAGATGCAGACCT |
Srsf12 | TTGGACTTCTACAGTCGCCG | ATTTGGCCTGGTGTTTTGCG |
Srsf1 promoter | CTCCCAAGCCTGACGTTTCA | CCCCACAGGTTCTAAGGGTG |
Bmf V1 | GCGGGCGTATTTTGGAAACA | CAGGGTCCAGGGTGAAGAAC |
Bmf V2 | AGCCTGGAGTTCAGACTTCG | TCTCCTGGGGACATAGAAGCA |
Bmf V3 | TGGCTGCTCTACAATTCCTG | GGCTCCATCTCTCTTCCTAAGC |
Bmf | AGCCTGGAGTTCAGACTTCG | TCTCCTGGGGACATAGAAGCA |
Bmf probe | ACAAAAGAGACTGATGAGAAAGGAC | GCCAGCTGCCTGAGATCC |
Foxc1 | CGCTTCAAGAAGAAGGACGC | GGACACGTACCGTTCTCCG |
Foxc1 (−2000 ~ −1801) | GCTGCGATCATCCTACCACA | TCTAGCCCAGCACACCAATG |
Foxc1 (−1800 ~ −1601) | AGAATCTTTAGGAACAACTGCTGAC | TGTGGGCTCACTTGATCCTCT |
Foxc1 (−1600 ~ −1401) | GAGAGAGAGTGTTGTGTTTGTGTG | CACCACCACCTCTCCCTTAGT |
Foxc1 (−1400 ~ −1201) | CCCAGCAAGTCCTAGCGTT | CACCAGGTCGTTTGCTAGAAG |
Foxc1 (−1200 ~ −1001) | GTTTTTCAGTCAGAGGGAGGC | GAATTGCACCTACAAGGCGG |
Foxc1 (−1000 ~ −801) | GAACACTTCCTCCAACTCAGACTC | TTGCCCCTCTGTCCTGACCA |
Foxc1 (−800 ~ −601) | TGTCGAGGAAACTTCTGGAGTC | AGATGGTATTTCGGTGCCTCG |
Foxc1 (−600 ~ −401) | GCCTTGGTAACCTAAAGTCGGA | ACTCTACCGTGCTGTCTCCA |
Foxc1 (−400 ~ −201) | GGCCTGGGAACAGTGGAC | GCACCCACCCACTCACTC |
Foxc1 (−200 ~ −1) | GCGCGCGTGTGGTCA | AACTTTTGAGCATCCGTCACC |
Hdac1 | GGACCGGTTAGGTTGCTTCA | TTCGTAAGTCCAGCAGCGAG |
Hdac2 | CTATCCCGCTCTGTGCCCTA | CCTCCTTGACTGTACGCCAT |
Hdac3 | GGTGGCTACACTGTCCGAAA | GGAGTGTGAAATCTGGGGCA |
Hdac4 | TCCAGCAGCGGATCTTGTTC | CTGTGACAAGGGGTGTCTGG |
Hdac5 | TGGCCTTGGATGGGCATTAG | CCGACTCGTTGGGAGAGTTC |
Hdac6 | AAATAGGCTGAAGCGGGCAG | GAAGAATCTTGGCCGGTGGA |
Hdac7 | AGCCCCAGTATTCCCTACAGA | AAAGTGTTCCGGGGGTTCAG |
Hdac8 | ATGTGCTGGAAATCACGCCA | CCGCTTGCATCAACACACTG |
Hdac9 | GTGACAGCAGTGCTTGTGTG | CCTGCTCCCCACATTCCATT |
Hdac10 | TAGTTAGGTGAGACCGGGCA | AATTTCGCACTCGGGGTCAT |
Hdac11 | CCCATCGTGTACTCACCACG | GTTGAGATAGCGCCTCGTGT |
Gapdh | CCCTTAAGAGGGATGCTGCC | TACGGCCAAATCCGTTCACA |
