Fig. 7: Gp78 E3 releases unanchored Ub3 chains from Ube2G2 in the presence of VHH12G2/G2BRs. | Communications Biology

Fig. 7: Gp78 E3 releases unanchored Ub3 chains from Ube2G2 in the presence of VHH12G2/G2BRs.

From: A nanobody that binds to the backside of the ubiquitin conjugating enzyme Ube2G2 differentially affects interactions with its partner E3 Ligases

Fig. 7

A–C Anti-ubiquitin Immunoblots of ubiquitination assays with E3 GST-Gp78 at indicated time points in the presence or absence of a 3-fold molar excess of (A) VHH12G2, (B) unrelated VHHSMT3, and (C) VHH12G2 or G2BR segments appended to SBP-His6 tags over Ube2G2. D Schematic of the Gp78 mechanism of action on Ube2G2 from reference31, along with the proposed explanation for the effect when VHH12G2 or isolated G2BR binds.

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