Fig. 6: l/vlPAG SST+ neurons project to the PMC. | Communications Biology

Fig. 6: l/vlPAG SST+ neurons project to the PMC.

From: SST neurons in the periaqueductal gray regulate urination and bladder function

Fig. 6

a Schematic of AAV-DIO-GFP injection into the unilateral l/vlPAG of SST-Cre mice (left). Representative image of GFP-labeled somas in the l/vlPAG (right). b, c These l/vlPAG SST+ neurons mainly sent descending outputs to the PMC, BNST, MPA, LPO, PVA, PVN, Zi, LHA, DMH, VTA, and RVM. n = 3 mice. d Schematic of AAV-Retro-DIO-Tdtomato injection into the unilateral PMC of SST-Cre mice. n = 3 mice. e, f Serial coronal brain slices from a single mouse with Tdtomato-labeled neurons in the whole l/vlPAG. g Summary of Tdtomato-labeled neurons in the ipsilateral l/vlPAG (Bregma: -4.24 to -4.84 mm, n = 3 mice). Black circles indicate the average number of Tdtomato-labeled l/vlPAG neurons in each brain section.

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