Fig. 4: H3K27me3 abundance differences in uninjured, wounded, and patterning competent limb tissues. | Communications Biology

Fig. 4: H3K27me3 abundance differences in uninjured, wounded, and patterning competent limb tissues.

From: Neural regulation of H3K27me3 during the induction of patterning competency in regenerating Axolotl limb cells

Fig. 4

a Sample generation scheme for H3K27me3 ChIP-sequencing analysis. b Heatmap of normalized enrichment scores of GO Biological Process Terms from Gene Set Enrichment Analysis (GSEA) in comparisons of H3K27me3 ChIP-seq data from Wound (W) relative to Mature (Mat.) and ND-A relative to Wound samples. Arrows indicate the direction of each comparison (refer to Supplementary Data 2.1). Data is segregated into four clusters (black circles 1–4) using K-means clustering. c Heat map of log2 fold change (log2FC) values of statistically significant genes that were pathway enriched in the GSEA. Limb patterning genes are indicated with blue font (refer to Supplementary Data 2.4). d Heatmap of log2FC values of limb patterning genes with statistically significant differences in H3K27me3 enrichment in each comparison (refer to Supplementary Data 2.5-2.6). e, f Heatmaps of log2FC values of cell cycle and immune and stress response-related genes with statistically significant differences in H3K27me3 enrichment in each comparison (full data sets and gene lists located in Supplementary Data 2.5 and 2.7-2.8). The top 2 most significant genes in each cluster are indicated in bold.

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