Fig. 5: Immune cells in the GL261-GSC model. | Communications Biology

Fig. 5: Immune cells in the GL261-GSC model.

From: Single-nucleus RNA sequencing reveals a preclinical model for the most common subtype of glioblastoma

Fig. 5

A UMAP visualization of immune cells, colored by Leiden cluster, time of tumor development, and cell type annotations. k = 2427 individual cells. B Barplot showing the proportion of cells at each time point per cluster. C Barplot showing the distribution of cells in each cell cycle phase at 7d or 28d. D UMAP visualization of microglia activation, microglia differentiation, microglia migration, and microglia phagocytosis scores. E Violinplots showing microglia activation, microglia differentiation, microglia migration, and microglia phagocytosis scores at 7 and 28 days. F Heatmap showing relative expression of each of the 50 top expressed genes in immune cells at 7 or 28 days. Columns correspond to cells, ordered by time. Selected genes are indicated (see Table S9 for the full list). G UMAP visualization of immune cells, colored by cell type annotations. H Stacked violinplot showing the expression of microglia and macrophage gene markers in 7d and 28d myeloid cells. I Barplot showing all 8 significantly overexpressed terms within the MSigDB Hallmark 2020 geneset in immune cells at 7 days and top 20 overexpressed terms at 28 days (see Tables S10, S11 for the full lists).

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