Fig. 2: The phylogenetic tree of the PUB gene family.

Multiple protein sequence alignments of the U-box domain sequences of Arabidopsis thaliana, Oryza sativa, M. acuminata, M. balbisiana, and Solanum lycopersicum were performed using the Clustal Omega multiple sequence alignment program, and a phylogenetic tree was constructed using the ggtree package in R.