Fig. 4: Sanger sequence analysis of pub22/23 edited events to detect mutations. | Communications Biology

Fig. 4: Sanger sequence analysis of pub22/23 edited events to detect mutations.

From: Loss of function of MusaPUB genes in banana can provide enhanced resistance to bacterial wilt disease

Fig. 4

a Mutations in targeting the MusaPUB22 gene. b Mutations in targeting the MusaPUB23 gene. Red nucleotides indicate the gRNAs, and purple nucleotides indicate the Protospacer Adjacent Motif (PAM). Black dashes (—) represent deletions, green nucleotides denote substitutions, and blue nucleotides indicate insertions. C, wild-type non-edited control sequences; P2-P50, independently edited events. The number of indels as deletions (‒), insertions (+), and substitutions (S) are indicated on the right panel. * indicates a big insertion in PUB22-25, and the sequence of the insert is written in blue. The first nucleotide in gRNA1 of MusaPUB22/23 is underlined to indicate the single-nucleotide polymorphisms (SNPs); ‘A’ in MusaPUB22 and ‘G’ in MusaPUB23.

Back to article page