Fig. 1: Domain organization of C. crescentus DriD.

A Schematic showing the 2-D domain organization of DriD and the constructs used in this study. The residue numbers encompassed within each domain is included with secondary structural elements shown over the schematics, where arrows represent ß sheets and coils represent α helices. The DNABD is in green (residues 1–70), the linker is red (residues 71–78), the 3HB is gray (residues 79–133), the WYL domain is purple (residues 134–245), and the WCX domain is blue (residues 246–327). Key residues that make contacts to target DNA and ssDNA are indicated with lines within the top schematic. The secondary structural elements above the domain schematics were generated in SSDraw⁶⁷. B Cartoon diagram of FL DriD (PDB: 8TP8) with domains colored according to Fig. 1A. Target double stranded DNA is shown in orange, and ssDNA is shown in black spheres. C A close-up view of the DriD-ssDNA interaction is shown. Residues that interact with ssDNA are shown in light purple sticks. ssDNA is shown in black sticks. D A close-up view of the DriD DNABD interacting with target DNA is shown. Key residues that contact the target DNA are shown in green sticks.