Fig. 4: HDX-MS analyses of DriD in the presence and absence of ligands.
From: Allosteric activation mechanism of DriD, a WYL-domain containing transcription regulator
A HDX-MS data for curated peptides. Peptides that are coincident across all the constructs of DriD FL studied (DriD FL, DriD FL + ssDNA, DriD FL + ssDNA + target DNA are shown. The relative difference data are calculated by subtracting the relative deuterium incorporation of the DriD-FL alone from the other constructs, and the differences are colored according to the scale shown. B Deuterium incorporation curves of selected peptides (indicated with a gray box in A labeled a–f). C The selected peptides are mapped on DriD FL (PDB ID: 8TP8) with respective color code labeled a–f. All HDX-MS data used to make the graphs as well as the complete HDX-MS difference data are found in Supplementary Data 1.
