Fig. 1: Mannose inhibits tumor growth in bladder cancer. | Communications Biology

Fig. 1: Mannose inhibits tumor growth in bladder cancer.

From: Mannose inhibits PKM2 lactylation to induce pyroptosis in bladder cancer and activate antitumor immune responses

Fig. 1

A Western blot analysis of PMI expression in urinary epithelial cells and bladder cancer cells (SVHUC1, 5637, T24, UMUC3). B Colony formation assay assessing the effects of mannose on the proliferation of urinary epithelial cells (SVHUC1) and cancer cells. C A CCK-8 assay was used to determine the duration of mannose treatment and the IC50 at 48 h, with values of ~45 mM for UMUC3 cells and 20 mM for 5637 cells. D Cell cycle analysis following mannose treatment in UMUC3 and 5637 cells. E PCR analysis of cyclin expression in UMUC3 and 5637 cells treated with mannose. F Flow cytometry analysis of UMUC3 and 5637 cell death induced by mannose treatment. G Assessment of apoptosis pathway activation in mannose-treated UMUC3 and 5637 cells. H Evaluation of the ability of mannose to inhibit the growth of bladder tumor organoids. I Assessment of the growth inhibition effect of mannose in nude mice bearing transplanted bladder tumors. J Immunohistochemistry (Ki-67 staining) confirming the inhibitory effect of mannose on bladder cancer tissue. K Detection of PARP cleavage in cells treated with mannose combined with cisplatin (CDDP) or pirarubicin (THP). L Analysis of the combined effects of mannose and cisplatin or pirarubicin on UMUC3 cells, with drug combination indices calculated via ZIP scores.

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