Fig. 1: Setup of the bone marrow model in the HUMIMIC Chip2 and preculture of mesenchymal stromal cells on the ceramic scaffold.

A Exploded view of the HUMIMIC Chip2 showing the three structural layers. B 2D view of the HUMIMIC Chip2 microfluidics: the outer compartment houses the bone marrow model; the inner compartment is used as a medium reservoir and as a sampling point for hematopoietic cells. C Calcein-AM RedOrange staining (red) of vital mesenchymal stromal cells populating the ceramic, scale is 250 µm. D The top 20 enriched GO BP terms in MSC samples from static and dynamic cultivation in MSC and HSC cultivation medium. Only GO BP terms from a list of GO biological processes significantly enriched among osteocyte transcriptome signature genes according to Youlten et al.²¹ were considered. E Dimensionality reduction of the gene expression profiles using PCA. The PCA is computed based on the top 500 most variable genes using TMM-normalized logCPM. Arrows indicate time course of the experiment. F GO biological processes enrichment analysis for differential expressed genes in different MSC culture conditions. Signatures correspond to rows and indications/contrasts to columns. The color shows the median log2 fold-changes of the genes in the signatures. Significance level is indicated with asterisks: *** 1% FDR, ** 5% FDR, *10% FDR.