Fig. 3: Characterization of the CD34 + HSPC starting cell population. | Communications Biology

Fig. 3: Characterization of the CD34 + HSPC starting cell population.

From: A microfluidic bone marrow chip for the safety profiling of biologics in pre-clinical drug development

Fig. 3

A Composition of starting material, percentages of CD38-Lin- stem cells, CD38+Lin- progenitor cells, CD71+ erythroid progenitor cells, CD33+ myeloid progenitor cells, CD10+ lymphoid progenitor cells and of CD34- differentiated cells are shown for 13 donors used throughout experiments. B Percentage of starting cell population of CD38+progenitor cells, CD71+ erythroid progenitor cells, CD33+ myeloid progenitor cells, CD10+ lymphoid progenitor cells. Values of 13 donors are plotted with mean and standard deviation. C Non-linear regression of CD10+ cells at day 0 of the assay and CD56 + NK-cell output at day 31 assuming exponential growth behavior. Individual NK-counts of three donors (N = 3) with three replicates (n = 3) are shown. D Non-linear regression of CD71+ cells at day 0 of the assay and CD45- erythroid cell output at day 31 assuming exponential growth behavior. Individual erythroid cell counts of five donors (N = 5) with three replicates (n = 3) are shown. E Non-linear regression of CD33+ cells at day 0 of the assay and CD14+ granulocyte output at day 31 assuming exponential growth behavior. Individual granulocyte counts of five donors (N = 5) with three replicates (n = 3) are shown.

Back to article page