Table 2 Flow cytometry panel used throughout the study
Laser | Emission detectors | Filters | Fluorophore | T cell co-culture allogenic setup + 1× TfR-binder study | T cell co-culture co-culture autologous setup + 2× TfR-binder study | Lineage differentiation and inflammatory factors+ 1× TfR-binder study | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|
Panel N° | 1. | 2. | 3. | 1. | 2. | 3. | 1. | 2. | |||
Violet 405nm | V1 | 450/50 | BV421 | CD19 | CD13 | CD69 | CD19 | CD13 | CD69 | CD14 | |
V2 | 525/50 | BV510 | CD45 | CD14 | HLA-DR | HLA-DR | CD19 | CD13 | |||
V3 | 579/34 | BV570 | L/D | L/D | |||||||
V4 | 615/20 | BV605 | CD45 | CD14 | |||||||
V5 | 667/30 | BV650 | CD34 | CD3 | CD3 | CD56 | CD34 | ||||
Blue 488nm | B1 | 525/50 | FITC | L/D | L/D | L/D | L/D | L/D + CD3 | L/D | CD45 | CD45 |
B2 | 579/34 | PE | CD235a | CD33 | CD25 | CD235a | CD45 | CD25 | CD235a | CD15 | |
B5 | 725/40 | PerCP/Cy5.5 | CD66b | CD33 | |||||||
B6 | 785/62 | PE-Cy7 | CD33 | CD16 | PD-1 | CD10 | CD16 | PD-1 | CD33 | CD33 | |
Red 640 nm | R1 | 667/30 | APC/AF647 | CD56 | CD15 | CD8 | CD56 | CD15 | CD8 | CD10 | CD16 |
R3 | 785/62 | APC-Cy7 | CD71 | CD41a | CD4 | CD71 | CD41a | CD4 | CD71 | CD41a | |
