Fig. 1: Proteomic landscapes of the male mouse heart.

a Graphical representation of the experimental workflow for the acquisition, identification, quantification, and bioinformatic analysis of mouse heart proteome, phosphoproteome, and S-nitrosocysteine proteomes. The figure was created using BioRender (license to Mohanty, I., 2024, https://biorender.com/z60z971), and the data images were generated from our proteomic analysis. b Histogram plot of the relative abundance of the 3,663 proteins quantified in all genotypes (purple) and 816 that are statistically different in the tNOS null mice, adjusted p-value < 0.05 (red). The proteome was acquired from n = 5 wild-type, n = 4 NOS1, n = 5 NOS2, n = 5 NOS3, n = 4 sGCα1 and n = 5 tNOS null mice. The gray bars represent the distribution of the manually curated and integrated reference mouse heart proteome of 7071 proteins. c Histogram of the relative abundance of phosphoproteins detected in all genotypes. 1064 proteins were identified only by the phosphorylation site (light green), whereas 1072 were detected in the proteome and phosphoproteome (blue). The phosphoproteome was acquired from n = 5 wild-type, n = 4 NOS1, n = 5 NOS2, n = 5 NOS3, n = 4 sGCα1 and n = 5 tNOS null mice. d Cumulative frequency histogram of the relative abundance of 415 S-nitrosylated proteins (red) in the wild-type mouse, n = 6.