Fig. 6: RBAP48 modulates the recruitment of WRAD subcomplexes to gene promoter regions. | Communications Biology

Fig. 6: RBAP48 modulates the recruitment of WRAD subcomplexes to gene promoter regions.

From: RBAP48 facilitates the oral squamous cell carcinoma process in an androgen receptor-dependent and independent manners

Fig. 6

A The pattern showed potential ARE sites in the promoter regions of CCND1 and RAB31 genes. B The pattern showed the potential SP1 binding sites (SPSs) in the promoter region of the RELA gene and the potential RELA binding sites (RSs) in the CCNE1 promoter. C–F The ChIP qPCR results revealed the recruitment of RBAP48, AR and ASH2L and the modification level of H3K4me3 at the ARE site after RBAP48 depletion treated with DHT in CAL-27 cells. The total amount of DNA measured by qPCR represented relative enrichment (%input), and the student t-tests were used. IgG represented the positive control of antibodies. G, H ChIP assays demonstrated the recruitment of RBAP48, AR and ASH2L, as well as changes in H3K4me3 modification levels in the SPS regions of (G) RELA promoter and RS regions of (H) CCNE1 promoter.

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