Fig. 5: High basal HSP in bats is not further transcriptionally induced during heat shock.

A Volcano plot of DEG of mice (Mm) and bats (Es) at baseline (Ctl) in lung and in (B) muscle (Supplementary Data 5). Coloured dots indicate different types of genes that are differentially expressed, Red – HSPs and grey – non HSP related genes. The number of DE upregulated HSP summarizes the total upregulated number of HSP in each species (Supplementary Data 5). C Heatmap of HSP family and co-chaperones baseline relative expression in each species. Z-score is derived via the scaled value of log(nCounts+1) normalized against each gene and organ (Supplementary Data 5). D Module signature of each HSP class across treatment conditions for mice (Mm) and bats (Es). The module signature is calculated from the arithmetic mean of log(nCounts+1) for all genes within the HSP family class listed in (C) Vertical line denotes standard error (Supplementary Data 5). E qPCR validation of HSPA1A and HSP90AA1 and (F) western blot of HSP70 and HSP90 in muscle tissue of animals that were subjected to in vivo heat shock (Supplementary Data 5). Figures were created with elements from BioRender (2025). https://BioRender.com/0fcvmt1.