Table 1 Cryo-EM data collection, refinement, and validation statistics for Fab HC84.26.5D–E2 complex
Fab HC84.26.5D–E2ecto1b09 | |||
|---|---|---|---|
Global refinement | Local refinement | Local refinement | |
Interface 1 | Interface 2 | ||
PDB | 8TGV | 8U9Y | 8TGZ |
EMDB | EMD-41245 | EMD-42041 | EMD-41247 |
Data collection and processing | |||
Microscope | Titan Krios | ||
Magnification | 81,000 | ||
Voltage (kV) | 300 | ||
Detector | Gatan K3 | ||
Camera mode | Counting | ||
Dose rate (e−/pixel/s) | 16.6 | ||
Total dose (e−/Å2) | 50 | ||
Exposure time (sec) | 3.8 | ||
Defocus range (µm) | −1.0 to −2.5 | ||
Pixel size (Å) | 1.12 | ||
Number of frames | 40 | ||
Movies collected | 9653 | ||
Refinement | |||
Micrograph selected | 8291 | ||
Software | cryoSPARC v4 | ||
Number of particles | 180,188 | 357,752 | 180,188 |
Map sharpening B factor (Å2) | −115.2 | −128.6 | −119.2 |
Map resolution (Å) (global) | 3.7 | 3.8 | 3.8 |
FSC threshold | 0.143 | 0.143 | 0.143 |
RMSD from ideality | |||
Bond lengths (Å) | 0.003 | 0.004 | 0.004 |
Bond angles (°) | 0.531 | 0.656 | 0.715 |
Clash score | 5.09 | 7.25 | 8.55 |
MolProbity score | 1.71 | 1.98 | 1.89 |
Number of residues | 2236 | 871 | 871 |
Ramachandran plot statistics | |||
Favored (%) | 93.2 | 90.5 | 93.4 |
Allowed (%) | 6.8 | 9.5 | 6.6 |
Outliers (%) | 0 | 0 | 0 |
Rotamer outliers (%) | 0.06 | 0.14 | 0.14 |
Mask CC | 0.80 | 0.75 | 0.77 |
Volume CC | 0.78 | 0.74 | 0.76 |
