Table 3 Cryo-EM data collection, refinement, and validation statistics for Fab CBH4B–E2 complex
Fab CBH4B–E2ecto1b09 | ||
|---|---|---|
Global refinement | Local refinement | |
PDB | 8TZY | 8TXQ |
EMDB | EMD-41774 | EMD-41703 |
Data collection and processing | ||
Microscope | Titan Krios | |
Magnification | 81,000 | |
Voltage (kV) | 300 | |
Detector | Gatan K3 | |
Camera mode | Counting | |
Dose rate (e−/pixel/s) | 18.0 | |
Total dose (e−/Å2) | 50 | |
Exposure time (s) | 3.5 | |
Defocus range (µm) | −1.0 to −2.5 | |
Pixel size (Å) | 1.17 | |
Number of frames | 40 | |
Movies collected | 7168 | |
Refinement | ||
Micrograph selected | 6317 | |
Software | cryoSPARC v4 | cryoSPARC v4 |
Number of particles | 200,763 | |
Map sharpening B factor (Å2) | −137.2 | −126.4 |
Map resolution (Å) (global) | 3.74 | 3.39 |
FSC threshold | 0.143 | 0.143 |
RMSD from ideality | ||
Bond lengths (Å) | 0.003 | 0.003 |
Bond angles (°) | 0.603 | 0.611 |
Clash score | 8.83 | 7.36 |
MolProbity score | 2.07 | 1.97 |
Number of residues | 978 | 610 |
Ramachandran plot statistics | ||
Favored (%) | 88.0 | 89.5 |
Allowed (%) | 12.0 | 10.5 |
Outliers (%) | 0 | 0 |
Rotamer outliers (%) | 0.41 | 0.00 |
Mask CC | 0.67 | 0.73 |
Volume CC | 0.65 | 0.71 |
