Fig. 2: Acute 24 hr exposure to BPA alters ON- and OFF-bipolar cell responses. | Communications Biology

Fig. 2: Acute 24 hr exposure to BPA alters ON- and OFF-bipolar cell responses.

From: Loss of adult visual responses by developmental BPA exposure is correlated with altered estrogenic signaling

Fig. 2

A–G Mean photopic electroretinogram (ERG) recordings from retinal eyecup preparations from adults exposed to water, vehicle (0.0003% DMSO), low BPA (0.001 µM), or high BPA (0.1 µM) for 24 hr. The tissue was stimulated by 300 msec full-field white light pulses at 7 irradiance levels ranging from ND 3.0 (brightest) - 6.0 (dimmest) in 0.5 increments. Traces shown in A represent the mean response to the brightest stimulus (3.0 ND) from all eye cups in each treatment group. The red square pulse denotes duration of the light stimulus. Water = 7 eyes; DMSO = 6 eyes; low BPA = 7 eyes; high BPA = 3 eyes. ERG a-wave, ON-bipolar cell b-wave, and OFF-bipolar d-wave components (noted on vehicle trace in (A)) were quantified. B a-wave amplitude and C a-wave trough time (p = 0.08) were not altered by BPA exposure. D b-wave amplitude was reduced following low BPA treatment (p < 0.001) though (E) time to b-wave peak was not changed (p = 0.162). Low BPA decreased d-wave amplitude (F) but increased (delayed) peak time (G) (p < 0.001). Data in B–G are presented as box and whisker plots: median line is in the center of the box and the ‘x’ is the mean. Significant differences are indicated with asterisks (α = 0.05).

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