Fig. 3: 24 hr exposure to BPA is sufficient for measurable accumulation in body tissues and to cause genomic changes. | Communications Biology

Fig. 3: 24 hr exposure to BPA is sufficient for measurable accumulation in body tissues and to cause genomic changes.

From: Loss of adult visual responses by developmental BPA exposure is correlated with altered estrogenic signaling

Fig. 3

Adult zebrafish were exposed to low BPA (0.001 µM), high BPA (0.1 µM), water, or vehicle (DMSO, 0.0003%) for 24 hr. A qPCR analysis of retinal homogenates tested if the 24 hr exposure regime altered expression of estrogen responsive (cyp19a1b, esr1, esr2a) and thyroid responsive (thrab, thrb) genes. Low BPA increased thrab expression compared to vehicle (p ≤ 0.01). White bars—water, light gray bars—vehicle, dark gray bars—low BPA, black bars—high BPA. B After removal of internal organs, carcasses were flash frozen for LC/MS analysis. Concentration dependent accumulation was evident, and no BPA was detected in either water or vehicle exposed carcasses. C Adults exposed to high BPA at either 72 hpf (light gray bars) or 7 dpf (dark gray bars) had measurable levels of BPA within their carcasses. BPA was not detected in adults developmentally exposed to low BPA at either age. No BPA was found in carcasses of adults that were developmentally exposed to either vehicle or water.

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