Fig. 9: Exposure to BPA at 72 hpf induced changes in genomic signaling pathways in adult retina. | Communications Biology

Fig. 9: Exposure to BPA at 72 hpf induced changes in genomic signaling pathways in adult retina.

From: Loss of adult visual responses by developmental BPA exposure is correlated with altered estrogenic signaling

Fig. 9

RT-qPCR and Western Blot results in retinal homogenates of adult fish exposed to water, vehicle (0.0003% DMSO), low BPA (0.001 µM), or high BPA (0.1 µM) when they were 72 hpf. A RT-qPCR of estrogenic genes cyp19a1b, esr1, and esr2a. N = 3-5 fish per treatment for each gene (cyp19a1b: vehicle = 3, High BPA = 3, Low BPA = 3, Water = 4, | esr1: vehicle = 4, High BPA = 5, Low BPA = 5, Water = 5 | esr2a: vehicle = 4, High BPA = 4, Low BPA = 4, Water = 4). B Western Blot using an antibody against ERβ and β-actin as a loading control and quantification. Fold changes were calculated from densitometry analysis and normalizing values to loading control and water. N = 2 fish per treatment. C RT-qPCR of thyroid receptor genes thrab and thrb. N = 3 fish per treatment for each gene. For RT-qPCR, values were normalized to rpl13a housekeeping gene and water control. For all graphs, box plots reflect fold change. Asterisks indicate statistical significance: *p < 0.05, **p < 0.01, ***p < 0.001.

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