Fig. 4: NHE7 accelerated the cell cycle progression, inhibited senescence-related proteins, and reduced SASP cytokine release in EC cells. | Communications Biology

Fig. 4: NHE7 accelerated the cell cycle progression, inhibited senescence-related proteins, and reduced SASP cytokine release in EC cells.

From: NHE7 drives endometrial cancer progression by delaying senescence through cAMP/CREB/GRIN2B axis-mediated Ca²⁺ influx

Fig. 4

A Flow cytometry was used to detect the effect of NHE7 on the cell cycle in HEC-1-A and Ishikawa cells. A value of p below 0.05 is considered significant, *p < 0.05, **p < 0.01. B β-galactosidase staining was used to detect the effect of NHE7 on senescence in HEC-1-A and Ishikawa cells (Scale bar: 50 μm). *p < 0.05, **p < 0.01. C WB assay was used to detect the expression of p53, p21, and p16 proteins in HEC-1-A and Ishikawa cells. *p < 0.05, **p < 0.01. D RT-qPCR assay was used to detect the mRNA expression of VEGF, IL-6, IL-1β, and MMP3 in HEC-1-A and Ishikawa cells. All data came from three repeated experiments (n = 3). *p < 0.05, **p < 0.01.

Back to article page