Fig. 4: Molecular characterization and herbicide resistance of PagALS base-edited poplar plants.
From: Synergistic optimization enhancing the precision and efficiency of cytosine base editors in poplar
a Representative sequencing chromatograms showing various Pro197 mutations in PagALS homologs. Mutations include amino acid substitutions, deletions, and premature stop codons. “Ref” indicates the reference sequence. b P197 amino acid changes across PagALS homologs (A01, A02, G01, G02) in different edited plant lines. Plants are grouped based on the number of successfully edited homologs. c Time-course comparison of the edited line 1#58 and wild-type (WT) potted plants treated with 3000 mg/L tribenuron. Images show plant status before treatment and 30 days after herbicide application, with close-up images of leaf morphology at the 30-day timepoint. Scale bar represents 10 cm. d In vivo herbicide resistance assay comparing growth of edited (1#58) and WT tissue-cultured seedlings after 30 days of culture on medium supplemented with 0.5 mg/L nicosulfuron. Terminal bud and stem segment growth are displayed. Scale bar represents 1 cm.
