Table 7 The editing efficiencies of hyPopCBE variants V1–V4, including the number of positive plants and effective biallelic and homozygous mutations

From: Synergistic optimization enhancing the precision and efficiency of cytosine base editors in poplar

Base editor

No. of positive plants

No. of effective biallelic mutation plants; percentage

No. of effective homozyous mutation plants; percentage

hyPopCBE-V1

43

12; 27.91%

2; 4.65%

hyPopCBE-V2

41

19; 46.34%

5; 12.20%

hyPopCBE-V3

43

13; 30.23%

3; 6.98%

hyPopCBE-V4

42

19; 45.24%

9; 21.43%

  1. “Effective” mutations refer to C to T conversions without any other byproduct edits. Homozygous editing indicates C to T conversions in all four homologous genes.
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