Fig. 1: Model hNPC line workflow and differentiation capability. | Communications Biology

Fig. 1: Model hNPC line workflow and differentiation capability.

From: Nascent actin dynamics and the disruption of calcium dynamics by actin arrest in developing neural cell networks

Fig. 1

Workflow for the proposed work (A), starting with time course of hNPC differentiating towards matured neurons and astrocytes, followed by analysis of calcium and actin dynamics. Cells are considered “naïve differentiated” at 4–10 days post-differentiation initiation and “mature differentiated” at 14–28 days post-differentiation initiation. Representative immunofluorescence image of undifferentiated hNPC (B) with expression of Nestin (green) and Sox2 (red). Immunofluorescence on naïve differentiated (4 days post-differentiation initiation) hNPC (C) with expression of TUBB3 (green) or GFAP (red) with nuclear staining (blue). Box plot representing the percentage of TUBB3 positive cells after 4 days differentiation (n = 8 biologically independent experiments; error bars represent the standard deviation across experiments) (D). Representative confocal fluorescent image of lifeact-GFP stable transduction of a mature differentiated (21 days post-differentiation initiation) cell derived from hNPC (E).

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