Fig. 4: Actin dynamics are generally oriented along the cell’s major axis and show a rhythmic character throughout differentiation. | Communications Biology

Fig. 4: Actin dynamics are generally oriented along the cell’s major axis and show a rhythmic character throughout differentiation.

From: Nascent actin dynamics and the disruption of calcium dynamics by actin arrest in developing neural cell networks

Fig. 4

Schematic with an arrow showing orientation along a cell’s major axis (A). Analysis from actin imaging captured at 0.25 or 0.125 Hz (n = 17). Representative cell samples under various stages of differentiation: Undifferentiated (B, G), naïve differentiated (C, H), and mature differentiated (D, I) showing either all vectors (top) or using vectors within the of top 15% magnitude (bottom). Rose plots (B–D) show probability of optical flow vector orientation over entire films. Dot plots of mean actin speed at various stages of differentiation using all vectors (E) and using vectors within the top 15% magnitude (F). Conditions marked with asterisk have significantly different means from the others (n = 17 time-lapse movies obtained across N = 3 biologically independent experiments; error bars represent the standard deviation across experiments). Kymographs (G–I) show directional probability over time of all vectors (top) or of the highest 15% magnitude vectors (bottom). Hotter colors indicate a higher probability.

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