Fig. 5: hNPC calcium dynamics can readily be quantified at the population level. | Communications Biology

Fig. 5: hNPC calcium dynamics can readily be quantified at the population level.

From: Nascent actin dynamics and the disruption of calcium dynamics by actin arrest in developing neural cell networks

Fig. 5

Microscope field of view of naïve differentiated (10 days post-differentiation initiation) hNPCs; scale bar representing 50 microns (A). Zoom in from (A), highlighting individual cell #151 in magenta; scale bar representing 50 microns (B). Trace of calcium fluorescent data over time from cell #151 (C). Kymograph representation of trace from C for cell #151; time on the x-axis, fluorescent intensity indicated as color (D). Automated cell centers are displayed as dots and numbered from the entire microscope field of view from (A); scale bar representing 50 microns (E). Kymograph representing all cell fluorescent intensities over time (F).

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