Fig. 7: Comparing the effects of excitable Tyrode’s solution and actin arrest on calcium dynamics.

Probability histograms of spike width and prominence of calcium spikes [~15,000 individual spikes, compiled from n = 14 time-lapse movies across N = 3–4 biologically independent experiments] in various conditions (A–C). Spike width and prominence of cells in medium with or without actin arrest (JLY) (A). Spike width and prominence of cells in either medium (Ctrl) or Tyrode’s solution (B). Spike width and prominence of cells in Tyrode’s solution with or without actin arrest (C). Conditions marked with asterisk are significantly different from one another (A–C). Dot plots showing the proportion of active cells (D) or spiking frequency (E) under tested conditions—control condition in medium, control condition in excitable Tyrode’s solution, actin arrest in medium, and actin arrest in excitable Tyrode’s solution (n = 1 4 time-lapse movies each with at least 50 cells per field of view, across N = 3–4 biologically independent experiments; error bars represent the standard deviation across experiments). Histograms showing the relative probability of max cross correlation values from all cells pairs under the same conditions as in (A and B) on a logarithmic y-axis (F). Schematic representation of calcium fluctuations in the context of conditions applied within this work (G). Conditions marked with asterisk are significantly different from one another.