Fig. 1: Expression pattern of CAPZA2 and establishment of model mice.
From: Deciphering the role of CAPZA2 in neurodevelopmental disorders: insights from mouse models
A, B Western blot representative bands and quantitative analysis describe the CAPZA2 levels at different developmental stages, from mouse embryos to postnatal stages (Male mice were selectively utilized upon confirmation of reliable sex determination, n = 3). C, D Western blot representative bands and statistical analysis of CAPZA2 in various brain regions of adult mice (5 months old, n = 3 male). E Representative coronal sections of WT mice. CAPZA2 expression in the brain was examined by immunofluorescence in WT mice (5 months old, n = 3 male). Scale bar = 500 μm, blue: DAPI; red: CAPZA2. F Schematic describing strategy to generate CAPZA2+/− and CAPZA2c.G776T/+ mice. G, H Western blot representative bands and quantification of CAPZA2 expression in the hippocampus and PFC of CAPZA2+/− and WT mice. n = 6 (WT/Het, 3 males and 3 females). Lane arrangement note: Blank lanes in Western blot indicate molecular weight marker positions. Uncropped original images are provided in the Supplementary Information. I The mRNA expression level of CAPZA2 in the hippocampus and PFC of CAPZA2+/− and WT mice. n = 6 (WT/Het, 3 males and 3 females). J, K Western blot representative bands and the quantification of CAPZA2 expression in the hippocampus and PFC of CAPZA2c.G776T/+ and WT mice. n = 5 (WT/MUT, 3 males and 2 females). Lane arrangement note: Blank lanes in Western blot indicate molecular weight marker positions. Uncropped original images are provided in the Supplementary Information. L The mRNA expression level of CAPZA2 in the hippocampus and PFC of CAPZA2c.G776T/+ and WT mice. n = 6 (WT/MUT, 3 males and 3 females). M Representative images of immunofluorescence staining for CAPZA2 in the hippocampus and PFC of CAPZA2+/- and WT mice (blue: DAPI; red: CAPZA2). N Representative images of immunofluorescence staining for CAPZA2 in the hippocampus and PFC of CAPZA2c.G776T/+ and WT mice (blue: DAPI; red: CAPZA2). O Significant differences in CAPZA2 fluorescence intensity in the hippocampus and PFC of mice in the CAPZA2+/− and WT groups. n = 6 (WT/Het, 3 males and 3 females). P Significant differences in CAPZA2 fluorescence intensity in the hippocampus and PFC of mice in the CAPZA2c.G776T/+ and WT groups. n = 6 (WT/MUT, 3 males and 3 females). Unpaired t test was performed for statistical analysis. The data represented as mean ± S.E.M; *P < 0.05, **P < 0.01, ***P < 0.001.
