Fig. 3: Key characteristics of synapses in the brains of CAPZA2+/− mice are altered.
From: Deciphering the role of CAPZA2 in neurodevelopmental disorders: insights from mouse models
A Representative confocal images (Golgi staining) of the pyramidal neuronal dendrites in the hippocampus and images of dendritic skeletonization obtained by imageJ analysis. (Scale bar, 50 μm.) B Sholl profile reporting almost no significant difference in dendritic branching complexity of hippocampal neurons in groups of CAPZA2+/− and WT mice. n = 15 neurons from 5 mice (WT/Het, 3 males and 2 females). C Schematic diagram of different morphology of dendritic spines. They can be categorized as filopodia spine, long-thin spine, thin spine, stubby spine, mushroom spine and branched spine, with two or more heads. Confocal micrographs of hippocampus dendrites from CAPZA2+/- and WT mice (D) and bar graphs with quantification of spine density (E) and spine morphology (F). n = 18 neurons from 6 mice (WT/Het, 3 males and 3 females). (Scale bar, 10 μm.) (G) Representative confocal images of the pyramidal neuronal dendrites in the PFC and images of dendritic skeletonization obtained by imageJ analysis. (Scale bar, 50 μm). H Sholl profile reporting significantly reduced dendritic branching complexity in the PFC of CAPZA2+/- mice. n = 15 neurons from 5 mice. (WT/Het, 3 males and 2 females). Confocal micrographs of PFC dendrites from CAPZA2+/- and WT mice (I) and bar graphs with quantification of spine density (J) and spine morphology (K). n = 18 neurons from 6 mice. (WT/Het, 3 males and 3 females, Scale bar, 10 μm.) L–N PSD95 representative immunoblots in CAPZA2+/− and WT mice, along with quantifying histograms and mRNA expression levels. n = 6 (WT/Het, 3 males and 3 females). Lane arrangement note: Blank lanes in Western blot indicate molecular weight marker positions. Uncropped original images are provided in the Supplementary Information. O–Q GRIN2A representative immunoblots in CAPZA2+/− and WT mice, along with quantifying histograms and mRNA expression levels. n = 6 (WT/Het, 3 males and 3 females). R–T GRIN2B representative immunoblots in CAPZA2+/− and WT mice, along with quantifying histograms and mRNA expression levels. n = 6 (WT/Het, 3 males and 3 females). U–Y GLUR2 and P-GLUR2 representative immunoblots in CAPZA2+/− and WT mice, along with quantifying histograms and mRNA expression levels. n = 6 (WT/Het, 3 males and 3 females). Two-way ANOVA and unpaired t test were performed for statistical analysis. The data represented as mean ± S.E.M; n.s. not significant. *P < 0.05, **P < 0.01, ***P < 0.001.
