Fig. 4: Nerve cell changes and their association with neurological dysfunction in CAPZA2+/- Mice.
From: Deciphering the role of CAPZA2 in neurodevelopmental disorders: insights from mouse models
A, B Representative fluorescence micrographs and statistical analysis of MAP2 showing the morphology and quantities of mature neuron in the hippocampus. (Enlarged image: Scale bar, 100 μm; Detailed image: Scale bar, 50 μm). n = 5 (WT/Het, 3 males and 2 females). C, D Representative fluorescence micrographs and statistical plot of DCX showing the morphology and quantities of neurons in the hippocampus. (Scale bar, 100 μm). n = 5 (WT/Het, 3 males and 2 females). E, F Representative fluorescence micrographs and statistical analysis showing the quantities of astrocytes the hippocampus. (Scale bar, 100 μm). n = 5 (WT/Het, 3 males and 2 females). G, H Western blot of GFAP in the hippocampus and PFC of CAPZA2+/− and WT mice with bands and statistical analysis. n = 6 (WT/Het, 3 males and 3 females). I The mRNA expression level of GFAP in the hippocampus and PFC of CAPZA2+/− and WT mice. n = 6 (WT/Het, 3 males and 3 females). J–L Representative fluorescence micrographs and statistical analysis showing the quantities of microglia in the hippocampus and PFC. (Scale bar, 100 μm). n = 5 (WT/Het, 3 males and 2 females). Unpaired t test were performed for statistical analysis. The data represented as mean ± S.E.M; n.s. not significant. *P < 0.05, **P < 0.01, ***P < 0.001.
