Fig. 5: Pathological similarities in CAPZA2c.G776T/+ and CAPZA2+/- mice highlight synaptic and neuronal disruptions.
From: Deciphering the role of CAPZA2 in neurodevelopmental disorders: insights from mouse models
A Representative confocal images of pyramidal neuronal dendrites in the hippocampus and images of dendritic skeletonization obtained by imageJ analysis. (Scale bar, 50 μm). B Sholl profile reporting almost no significant difference in dendritic branching complexity of hippocampal neurons in groups of CAPZA2c.G776T/+ and WT mice. n = 15 neurons from 5 mice (WT/MUT, 3 males and 2 females). Confocal micrographs of hippocampal dendrites from CAPZA2c.G776T/+ and WT mice (C) and bar graphs with quantification of spine density (D) and spine morphology (E). n = 18 neurons from 6 mice (WT/MUT, 3 males and 3 females, Scale bar, 10 μm). F Representative confocal images of pyramidal neuronal dendrites in the PFC and images of dendritic skeletonization obtained by imageJ analysis. (Scale bar, 50 μm). G Sholl profile reporting significantly reduced dendritic branching complexity in the PFC of CAPZA2c.G776T/+ mice. n = 15 neurons from 5 mice. (WT/MUT, 3 males and 2 females). Confocal micrographs of PFC dendrites from CAPZA2c.G776T/+ and WT mice (H) and bar graphs with quantification of spine density (I) and spine morphology (J). n = 18 neurons from 6 mice (WT/MUT, 3 males and 3 females, Scale bar, 10 μm). K, L Representative fluorescence micrographs and statistical analysis of MAP2 showing the morphology and quantities of mature neuron in the hippocampus. (Enlarged image: Scale bar, 100 μm; Detailed image: Scale bar, 50 μm). n = 5 (WT/MUT, 3 males and 2 females). M, N Representative fluorescence micrographs and statistical analysis of DCX showing the morphology and quantities of neurons in the hippocampus. (Scale bar, 100 μm). n = 5 (WT/MUT, 3 males and 2 females. O, P Representative fluorescence micrographs and statistical analysis showing the quantities of astrocytes in the hippocampus. (Scale bar, 100 μm). n = 5 (WT/MUT, 3 males and 2 females). Q Western blot with bands and statistical analysis and the mRNA expression level of GFAP in the hippocampus and PFC of CAPZA2c.G776T/+ and WT mice. n = 6 (WT/MUT, 3 males and 3 females). R–T Representative fluorescence micrographs and statistical plot showing the quantities of microglia in the hippocampus and PFC. (Scale bar, 100 μm). n = 5, (WT/MUT, 3 males and 2 females). U PSD95 representative immunoblots in CAPZA2c.G776T/+ and WT mice, quantifying histograms as well as mRNA expression levels. n = 6 (WT/MUT, 3 males and 3 females). V GRIN2A representative immunoblots in CAPZA2c.G776T/+ and WT mice, quantifying histograms as well as mRNA expression levels. n = 6 (WT/MUT, 3 males and 3 females). W GRIN2B representative immunoblots in CAPZA2c.G776T/+ and WT mice, quantifying histograms as well as mRNA expression levels. n = 6 (WT/MUT, 3 males and 3 females). X GLUR2 and P-GLUR2 representative immunoblots in CAPZA2c.G776T/+ and WT mice, quantifying histograms as well as mRNA expression levels. n = 6 (WT/MUT, 3 males and 3 females). Two-way ANOVA and unpaired t test were performed for statistical analysis. The data represented as mean ± S.E.M; n.s. not significant. *P < 0.05, **P < 0.01, ***P < 0.001.
