Fig. 6: Single-nucleus transcriptome analysis of the hippocampus in CAPZA2+/− mice.
From: Deciphering the role of CAPZA2 in neurodevelopmental disorders: insights from mouse models
A Single-nucleus transcriptome profiling workflow. B Number of seven cell types detected by transcriptome sequencing. UMAP plot (C) and bar plot (D) showing the proportions of the seven major cell types found in the CAPZA2+/− and WT mice of hippocampus. E Venn diagram showing the 19 coregulated DEGs among all seven cell types. The numbers of DEGs specific to each cell type are also presented. F Volcano diagram showing differentially expressed genes in seven cell types. G, H Gene ontology analysis of the top 10 functional clusters of up-regulated. G and down-regulated (H) differentially expressed genes in the hippocampus of CAPZA2+/− compared to WT mice. I, J Decreased mRNA expression levels of differently expressed genes in the hippocampus in CAPZA2+/− and CAPZA2c.G776T/+ mice compared with WT mice. K, L Increased mRNA expression levels of differently expressed genes in the hippocampus in CAPZA2+/− and CAPZA2c.G776T/+ mice compared with WT mice. n = 6 (WT/Het/MUT, 3 males and 3 females). Unpaired t test was performed for statistical analysis. The data represented as mean ± S.E.M; *P < 0.05, **P < 0.01, ***P < 0.001.
