Fig. 3: Growth curves of E. coli transformed with C. innocuum or E. coli alsE.
From: Gut microbial utilization of the alternative sweetener, D-allulose, via AlsE
A, B Verification of the Escherichia coli alsE functionality. Keio ΔalsE::E. coli alsE is the growth curve of the transformed Keio E. coli containing the wild-type E. coli alsE with IPTG to induce ectopic expression, and without IPTG, resulting in no gene expression. pCW-lic-E. coli_alsE is the growth curve of the transformed pCW-lic vector, containing the wild-type E. coli alsE with IPTG to induce ectopic expression, and without IPTG, resulting in reduced gene expression. C, D Verification of the Clostridium Innocuum 6_1_30 alsE functionality. Keio ΔalsE::C. Innocuum alsE is the growth curve of the transformed Keio E. coli containing the C. innocuum alsE with IPTG to induce ectopic expression, and without IPTG, resulting in reduced gene expression. pCW-lic_C.Inn_alsE is the growth curve of the transformed pCW-lic vector, containing the C. innocuum alsE with IPTG to induce ectopic expression, and without IPTG, resulting in reduced gene expression. The error bars correspond to the standard error of OD600 on n = 3 technical replicates.
