Fig. 5: Delineation and structural analysis of AlsE.
From: Gut microbial utilization of the alternative sweetener, D-allulose, via AlsE
A Gene tree constructed from putative AlsE sequences and related enzymes annotated as pentose-5-phosphate-3-epimerase (COG0036), showing a possible delineation of the AlsE clade and the location of Klebsiella pneumoniae, Clostridium innocuum, and Escherichia coli AlsE. B Diagram showing the change and conservation (entropy) of residues in the putative AlsE clade, as well as the GRASP predicted ancestral states of N129 (AlsE clade) and N91 (putative ancestral node). Residues with a predicted conserved change from the ancestral state are labeled with a star. C D-allulose docked to the AlphaFold2-predicted structure of C. innocuum 6_1_30 AlsE, colored by amino acid conservation via Consurf.
