Fig. 3: Intestinal FXR participates in the occurrence and development of AAD by promoting the synthesis of ceramides. | Communications Biology

Fig. 3: Intestinal FXR participates in the occurrence and development of AAD by promoting the synthesis of ceramides.

From: Ursodeoxycholic acid alleviates aortic aneurysm and dissection through the intestinal farnesoid X receptor/ceramide synthase 2 axis

Fig. 3

A Differential lipid heatmap of ileal tissue. (n = 6 per group). B Kyoto Encyclopedia of Genes and Genomes (KEGG) Bubble Chart for inter group differential metabolites (n = 6 per group). C Quantification of serum ceramides in normal mice, AAD mice and UDCA mice (n = 8 per group). D Pearson’s correlation analysis between serum FGF15 and C20 ceramide levels in mice. E–G Detection results of ceramide synthase expression in the AAD model induced by BAPN. (n = 6, per group). H–J Detection results of ceramide synthase expression in the AAD model induced by AngII. (n = 6, per group) (K–L) Western blot results confirm that treatment with the FXR agonist GW4064 (10 μM) or DMSO enhances the expression of Cers2 in primary enterocytes (n = 4 per group). M Motif analysis shows FXR putatively bound to Cers2 motif. N Chip-qPCR was performed in mice treated with GW4064 (80 mg/kg/d) or DMSO (n = 3 per group). O Results of the dual-luciferase assay (n = 3 per group). Data in L and N are shown as the mean ± SEM. Statistical analysis was performed using two-tailed unpaired Student’s t test. Data in (C, E–J, O) are shown as the mean ± SEM. Statistical analysis was performed using a one-way anova test with Dunnett’s multiple comparisons posttest. (*p < 0.05, **p < 0.01, ***p < 0.001).

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