Fig. 2: MEN1 depletion impaired decidualization and led to increased proliferation of stromal cells. | Communications Biology

Fig. 2: MEN1 depletion impaired decidualization and led to increased proliferation of stromal cells.

From: Endometrial stromal Menin supports endometrial receptivity by maintaining homeostasis of WNT signaling pathway through H3K4me3 during WOI

Fig. 2

a qRT-PCR analysis of MEN1, PRL, and IGFBP1 mRNA expression in human endometrial stromal cells (hESCs) treated with E2, MPA, and 8Br-cAMP for 0, 2, and 4 days (n = 3). b ELISA analysis of the secreted levels of PRL in spent medium at D0 and D4 of decidualization in the shCtrl and shMEN1 groups (n = 3). c Western blotting assay of Menin and IGFBP1 protein expression in the shCtrl and shMEN1 groups treated with E2, MPA, and 8Br-cAMP for 4 days (n = 3). d Immunofluorescence (IF) staining of F-actin in primary hESCs transfected with shCtrl or shMEN1 following 96 h of EPC treatment (n = 3). e Volcano plot of differentially expressed genes detected by RNA sequencing (RNA-seq) in hESCs decidualized for 4 days with shCtrl and shMEN1. f Heatmap of decidualization-related genes in the shCtrl and shMEN1 groups treated with E2, MPA, and 8Br-cAMP for 4 days (n = 3) as determined by RNA-seq. g qRT-PCR analysis of HAND2, FST, CEBPB, and EGR1 expression in the shCtrl and shMEN1 groups treated with E2, MPA, and 8Br-cAMP for 4 days (n = 3). h IF staining for EdU in the shCtrl and shMEN1 groups and EdU-positive proportion (%) of the shCtrl and shMEN1 groups (n = 3); scale bar = 200 µm. i CCK-8 assay of the shCtrl and shMEN1 groups treated with E2, MPA, and 8Br-cAMP for 0 and 2 days (n = 4). Data are expressed as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, Student’s t test, one-way ANOVA and two-way ANOVA.

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